FPTASE, GST-FUSION, HISTAG, RAT, RECOM

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
产品信息
Form  Liquid
Formulation  In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Molar mass  120000
Avoid freeze/thaw  是的
Biological activity  1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Purity  ≥90% by SDS-PAGE
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling