C9-DEPLETED SERUM, HUMAN

Complement C9-Depleted Serum, Human
Human serum depleted of complement component C9 by affinity chromatography.
产品信息
Form  Liquid
Formulation  In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.
Preservative  None
Avoid freeze/thaw  是的
Unit definition Classical Pathway Activity: One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 107EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Source  Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling