C1Q-DEPLETED SERUM, HUMAN

Complement C1q-Depleted Serum, Human
Human serum that has been depleted of C1q complement component.
产品信息
Form  Clear, amber solution
Formulation  In PBS, ≤0.1% sodium azide, pH 7.4.
Preservative  None
Avoid freeze/thaw  是的
Unit definition Classical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Contaminants  C1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
Source  Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling