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Chitosanase, Streptomyces sp. N174
Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
产品信息
Form Liquid
Formulation In 50 M sodium acetate, 50% glycerol, pH 5.0.
Molar mass 29500
Avoid freeze/thaw 是的
Specific activity ≥25 units/mg protein
Unit definition One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
Contaminants chitinolytic activity: ≤0.001%, proteases: ≤0.01%
pH value optimum 5.5
PI 7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
EC number 3.2.1.132
CAS number 51570-20-8
商店和运输信息
Storage -20°C
Ship Blue Ice Only
Standard Handling |