CATHEPSIN V, HISTAG, HU., REC., NSO CELL

Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells
Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
产品信息
Form  Frozen liquid
Formulation  In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
Molar mass  38700
Avoid freeze/thaw  是的
Specific activity  ≥300 units/µg
Unit definition  One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
Purity  ≥95% by SDS-PAGE
Contaminants  Endotoxins: ≤1.0 EU/µg Cathepsin V
EC number  3.4.22.43
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling