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Casein Kinase II, α-Subunit, Human, Recombinant, E. coli
Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity. Note: 1 mU = 1 milliunit.
产品信息
Form Liquid
Formulation In 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
Molar mass 45100
Specific activity ≥1.0 unit/mg protein
Unit definition One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
Purity ≥95% by SDS-PAGE
Contaminants slight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |