BCL-2 CONVERTER,D-NUBCP-9-R8,CELL-PERMEA

Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable
(D)-Ac-fsrslhsll-G-NH(CH2)5-CO-rrrrrrrr-NH2, D-NuBCP-9
An all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
产品信息
Peptide Sequence  (D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH2)5-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH2
Form  Lyophilized solid
Formulation  Supplied as a trifluoroacetate salt.
Molar mass  2520.
Hygrocopic  是的
Packaged under inert gas  是的
Purity  ≥95% by HPLC
Solubility  H2O or DMSO
Chemical formula  C106H191N49O23
商店和运输信息
Storage  +2°C to +8°C
Ship  Ambient Temperature Only
Standard Handling