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Transcriptor Reverse Transcriptase
A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.
Contents
1.Transcriptor Reverse Transcriptase, in storage buffer
2.Transcriptor RT buffer, 5x conc.
Application
Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications:
•Synthesis of first-strand cDNA for use in subsequent amplification reactions
•RT-PCR of GC-rich RNA templates
•Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
•Retrieving and cloning the 5' and 3' termini of mRNA by RACE
•Generation of cDNA libraries with large inserts
•Dideoxy DNA sequencing
•RNA sequencing
•3'-end labeling of DNA fragments
•Generation of single-stranded probes for genomic footprints
Benefits
•Achieve high sensitivity in two-step RT-PCR.
Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments).
•Obtain more full-length transcripts - up to 14 kb.
cDNA libraries with large inserts can be generated.
•Reverse transcribe difficult templates.
The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions.
•Efficiently label cDNA.
Cy3-, Cy5-, DIG-, biotin-, or aminoallyl-labeled nucleotides are incorporated during cDNA synthesis.
Product Description
Volume activity:
20 U/µl. |