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Transcriptor One-Step RT-PCR Kit
For hot start one-step RT-PCR, utilizing the enzyme mixture of Transcriptor Reverse Transcriptase, Protector RNase Inhibitor, and the Expand System, with an innovative hot start buffer (patents pending).
Contents
1.RT-PCR Enzyme Mix containing Transcriptor Reverse Transcriptase, Protector RNase Inhibitor, and the Expand System
2.RT-PCR Reaction Buffer containing hot start mediating components and dNTPs – 5x concentrated
3.Water, PCR-grade
4.Control RNA – HAV RNA , in vitro transcript *
5.Control primer mix HAV RNA (forward and reverse primer) *
* Included only in the 50-reaction pack size.
Application
The Transcriptor One-Step RT-PCR Kit is designed for fast, sensitive, and specific end-point RT-PCR analysis of RNA (total RNA, mRNA, in vitro-transcribed RNA, or viral RNA) using gene-specific primers.
Featuring an innovative reaction buffer, the kit combines sensitive, high-yield reverse transcription with the improved performance of a hot start system for high-fidelity, high-yield amplification of a variety of templates, including GC-rich RNA.
The kit offers a convenient, highly sensitive one-step RT-PCR method to generate transcripts up to 6.5 kb for applications such as:
•Qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels
•Determining the presence or absence of RNA templates
•Quantifying gene expression through RNA analysis (e.g., for virus-level quantification)
•Cloning of RNA up to 6.5 kb
The one-step format is convenient, reduces reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps.
Benefits
•Obtain high sensitivity and yield in a wide amplification range:
Detect as little as 1 fg of total RNA (Figures 1 and 2).
•Achieve high specificity and reduce primer-dimers:
Count on excellent performance with the kit’s proprietary hot start buffer (patents pending, Figure 2).
•Produce long fragments:
Generate transcripts up to 6.5 kb (Figure 3).
•Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA):
Overcome high secondary structure with thermostableTranscriptor Reverse Transcriptase in combination with an optimized buffer (Figure 1).
•Save time and improve results with a convenient kit:
Minimize pipetting steps and prevent contamination by using a premixed enzyme blend and optimized buffer that includes PCR-grade dNTPs and hot start components.
•Protect your RNA sample:
Safeguard your RNA from degradation by RNases with the supplied thermostable Protector RNase Inhibitor that is functional during one-step RT-PCR when other RNase inhibitors fail.
•Increase confidence in your results:
Use the supplied control reactions to monitor performance.
Product Description
The Transcriptor One-Step RT-PCR Kit provides all components required for one-step RT-PCR (except primers and template) in a convenient format. The 50-reaction pack size also includes 10 control reactions (control RNA and control primer mix).
The kit’s RT-PCR Enzyme Mix contains four different enzymes: Transcriptor Reverse Transcriptase ensures sensitive and robust reverse transcription with high yield; Protector RNase Inhibitor, fully active at elevated temperatures, provides maximum template protection during reverse transcription; and the Expand System – a blend consisting of Taq DNA Polymerase and a proofreading polymerase – minimizes the possibility of mutations, offering high yield and fidelity in the PCR.
The optimized RT-PCR Reaction Buffer includes high-quality dNTPs and provides the overall improved performance of a hot start system. The buffer’s unique hot start component binds and sequesters primers at lower temperatures to prevent the p |