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X-tremeGENE siRNA Transfection Reagent
High-efficiency reagent for the transfection of siRNA into animal cells.
Benefits
•Transfects siRNA very efficiently. See "Typical Experiment" below for proof.
•Works on many different cell types. To see an up-to-date list of cells that have been successfully transfected with X-tremeGENE siRNA Transfection Reagent , visit www.roche-applied-science.com/transfection.
•Promotes effective gene knockdown. Short interfering RNA, transfected with X-tremeGene siRNA transfection reagent, has knocked down gene expression over 90% in many different cell types.
•Maximizes experimental flexibility. You can use a single reagent for siRNA- and co-transfection-based gene-knockdown experiments.
•Produces meaningful results. The low cellular cytotoxicity of X-tremeGene siRNA transfection reagent ensures that the cellular effects you observe are due to the transfected siRNA rather than the transfection procedure.
•Easy to use. The transfection procedure involves just two easy steps: (1) Mixing and incubation of diluted transfection reagent with diluted siRNA for complex formation; and (2) addition of complex to cells.
•Saves time. X-tremeGENE siRNA Transfection Reagent works well in the presence or absence of serum. Thus, transfection with this reagent does not require medium changes (e.g., to serum-free medium) before or after transfection.
Product Description
Composition: X-tremeGENE siRNA Transfection Reagent is a proprietary blend of lipids and other components, free of animal products, in a sterile-filtered solution.
Background Information
Short interfering RNA (siRNA) can be directly introduced into cells by transfection. A lipid-based transfection reagent, such as X-tremeGENE siRNA Transfection reagent, can provide a convenient, reliable, and efficient vehicle for delivering siRNAs into animal cells. Thus, X-tremeGENE siRNA Transfection Reagent from Roche Applied Science can help you study the cellular and functional consequences of gene knockdown. (See "Typical Experiments" below).
Note: For an overview of all the products that RAS offers for gene knockdown experiments, visit our Gene Knockdown Special Interest Site: www.roche-applied-science.com/geneknockdown.
Contents
Sterile-filtered solution, suppled in polypropylene tubes.
Application
X-tremeGENE siRNA Transfection Reagent is an optimized lipid-based transfection reagent that forms a complex with Short interfering RNA (siRNA) and with mixtures of siRNA and plasmid DNA. The transfection complex can directly and efficiently introduce siRNA into animal cells to induce gene silencing.
Note: X-tremeGENE siRNA Transfection Reagent efficiently delivers siRNA into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines such as HT29, a human adenocarcinoma cell line. (See "Typical Experiment" below.)
To see an up-to-date list of cells that have been successfully transfected with X-tremeGENE siRNA Transfection Reagent , visit our Transfection Special Interest Site: www.roche-applied-science.com/transfection . At the same site, you can also learn more about this reagent.
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