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PCR DIG Labeling Mix
For direct labeling of amplification products with digoxigenin-dUTP in the polymerase chain reaction (PCR).
Pack size:500 µl (2 x 250 µl) for 2 x 25 PCR assays Reactions of 100 µl final reaction volume (final concentration 200 μM).
Application
For monitoring the polymerase chain reaction.
The PCR DIG labeling mix can replace the unlabeled nucleotide mix in PCR. The nucleotide concentration ensures maximum yield of PCR product combined with DIG detection, with only a minor influence on the molecular weight of the product.
Note: This product is not a direct replacement for the mix included in the PCR DIG Probe Synthesis Kit.
Product Description
Labeling efficiency: 10-5µl of the PCR assay can be detected in a spot test with anti-DIG-AP and chromogenic or chemiluminescent substrates.
Contents
10x solution with: 2 mM dATP, dCTP, dGTP (each), 1.9 mM dTTP, 0.1 mM DIG-11-dUTP in 2 x 250 µl double-distilled water, pH 7.0.
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