mini Quick Spin RNA Columns Application Quick and complete removal of label Use mini Quick Spin RNA Columns to remove unincorporated nucleotides from freshly prepared, radiolabeled RNA (≥ 20 bases). Note: Typical recovery of RNA is ≥80%. Benefits Saves time and effort; mini Quick Spin Columns can be used in any standard microcentrifuge. Purifies more nucleic acid in less time; the columns can process up to 75 μl sample in only 7 min. Minimizes sample loss while maximizing yield; the two-step procedure is short, but effective. Easy to handle; the column comes with a cap for rapid resuspension of the gel matrix and a bottom tip that can be snapped off. Tested to ensure consistent results; quality testing ensures the columns retain unincorporated nucleotides while delivering high yields of labeled RNA. Product Description Brief description: Pre-packed, pre-swollen, microfuge-compatible columns, with a cap and snap-off bottom tip Recovery of labeled RNA: ≥80% Retention of unincorporated nucleotides: >99% Sample size: 20 – 75 µl Contents Suspension of Sephadex® G-50 in STE buffer (10 mM Tris-HCI, pH 7.5, 1 mM EDTA, 100 mM NaCl). |