M30 Cytodeath, 250 tests

Product Description
Assay time: 2 hours for immunofluorescence on cells, 3.5 hours for staining of tissues (excluding dewaxing)
Sample material: Adherent cells, tissue samples (routinely fixed and paraffin-embedded tissue sections, cryostat sections)
Specificity: The M30 CytoDEATH antibody binds to a caspase-cleaved, formalin-resistant epitope of the cytokeratin 18 (CK 18) cytoskeletal protein. The immunoreactivity of the antibody is confined to the cytoplasm of apoptotic cells.
Background Information
During apoptosis, vital intracellular proteins are cleaved. The proteases that mediate this process are called caspases (Cysteinyl-aspartic acid proteases). Caspases are expressed as zymogenes, which are activated by different apoptosis inducers. Once activated, a single caspase activates a cascade of caspases.
Until recently, cytokeratins, in particular cytokeratin 18, were not known to be affected by early events of apoptosis. Recently, it has been shown that the M30 antibody recognizes a specific caspase-cleavage site within cytokeratin 18, which is not detectable in native CK18 of normal cells. Consequently, the M30 CytoDEATH antibody is a unique tool for the easy and reliable determination of very early apoptotic events in single cells and tissue sections.
Contents
Mouse monoclonal antibody (clone M30), lyophilized, stabilized, formalin grade.
Application
Use the M30 CytoDEATH antibody for the determination of early apoptosis in cells and tissue sections. A specific epitope of cytokeratin 18, which is presented after cleavage by caspases, is detected.
Benefits
Convenient: Easy-to-use standard protocol
Clear results: Apoptotic cells are most easily distinguishable from normal cells
Early detection of apoptosis: Detects caspase-cleaved Cytokeratin 18; caspase activity is one of the earliest apoptosis markers
Useful for paraffin-embedded tissue: Useful for routinely fixed tissue samples; retrograde studies are possible