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High Pure RNA Tissue Kit
Low to medium throughput RNA isolation
Application
Use this High Pure kit to isolate total RNA from mammalian tissues. The isolated RNA can be used for many common molecular biology techniques, such as:
•RT-PCR
•Northern blotting
•RACE
•Primer extension
•Differential display
•cDNA library construction
•RNase protection assay
•In vitro translation
Benefits
•Isolates total RNA from a variety of tissues
•Prepares RNA samples in approx. 30 minutes
•Supplies RNA that is suitable for many downstream applications
•Minimizes RNA loss by eliminating time-consuming procedures such as precipitation and solvent extraction
Product Description
Sample
Solid tissue (e.g., mouse liver, spleen, lung, heart): 1 - 25 mg
Note: Sample size depends, in part, on the method used to prepare the tissue for the procedure; larger samples (>10 mg) should be processed via rotor-stator homogenization.
Background Information
Nucleic acids (NA) bind to the surface of glass fiber fleece in the presence of chaotropic salt. This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. When the desired product is RNA, the binding conditions can be optimized to ensure immobilization of all the RNA.
Contents
1.Lysis/Binding Buffer, 25 ml
2.DNase I, lyoph., 10 kU
3.DNase Incubation Buffer, 10 ml
4.Wash Buffer I, 33 ml
5.Wash Buffer II, 10 ml
6.Elution Buffer, 30 ml
7.High Pure Spin Filter Tubes (containing glass fiber fleece), 50
8.Collection Tubes (2 ml), 50 |