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High Pure RNA Isolation Kit
Low to medium throuhgput, mini scale RNA isolation.
Contents
1.Lysis/Binding Buffer, 25 ml
2.DNase I, recombinant, lyoph., 10 kU
3.DNase Incubation Buffer, 10 ml
4.Wash Buffer I, 33 ml
5.Wash Buffer II, 10 ml
6.Elution Buffer, 30 ml
7.High Pure Filter Tubes, one bag; 50 polypropylene tubes containing two layers of glass fiber fleece which can hold up to a 700-μl sample volume
8.Collection Tubes, one bag; 50 polypropylene tubes (2 ml)
Application
Use this High Pure kit to rapidly isolate small amounts of total RNA from a variety of samples ( e.g ., mammalian cells, blood, yeast, bacteria). The isolated RNA is ideal for many downstream molecular biology techniques, such as:
•RT-PCR
•Northern blotting
•RACE
•Primer extension
•Differential display
•cDNA library construction
•RNase protection assay
•In vitro translation
Benefits
•Quickly process multiple samples.
A single sample preparation is completed in 25 minutes, and multiple samples can be prepared in 45 minutes.
•Prevent RNA loss.
Precipitation or solvent extraction steps are not required.
•Avoid DNA contamination.
Integrated DNA digestion and DNase removal eliminates interference from genomic DNA.
•Obtain concentrated RNA.
RNA is eluted in a 50 μl volume.
Product Description
Sample
Cells in culture: 106
Human blood: 200 - 500 μl
Yeast: 108 cells
Bacteria (gram positive or gram negative): 109 cells |