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High Pure PCR Product Purification Kit
High Pure Plasmid Isolation Kit
Low to medium throughput, mini scale, plasmid isolation.
Contents
1.Suspension Buffer
2.RNase A, dry powder
3.Lysis Buffer
4.Binding Buffer
5.Wash Buffer I
6.Wash Buffer II
7.Elution Buffer
8.High Pure Spin Filter Tubes (containing glass fiber fleece)
9.Collection Tubes, 2 ml
Application
Use this High Pure kit to prepare up to 15 µg purified plasmid DNA from bacterial cultures, so it may be used directly for:
•PCR
•Sequencing
•Cloning
•In vitro transcription
•Generation of labelled hybridization probes
Benefits
•Quickly purifies up to 24 plasmid samples in <30 min
•Reduces handling steps, minimizing the loss and fragmentation of DNA
•Makes DNA suitable for immediate downstream processing
•Allows quantitative removal of contaminants, to improve the reliability and reproducibility of downstream procedures
Product Description
Sample: 0.5 - 4.0 ml cultures of E. coli (harvested at a density of 1.5 - 5.0 A600 units per ml) |