|
FastStart Taq DNA Polymerase, dNTPack 5 U/µl
Thermally activated polymerase for hot start PCR amplification of genomic and cDNA targets up to 3 kb.
Contents
1.FastStart Taq DNA Polymerase, 5 U/µl in storage and dilution buffer
2.PCR Reaction Buffer, 10x conc. with 20 mM MgCl2
3.PCR Reaction Buffer, 10x conc. without MgCl2
4.MgCl2 Stock Solution, 25 mM
5.GC-RICH Solution, 5x conc.
6.PCR Grade Nucleotide Mix
Application
FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C.
•PCR
•Hot Start PCR
•RT-PCR
Benefits
•Insist on higher specificity, sensitivity, and yield.
Hot start PCR dramatically improves the overall PCR performance.
•Challenge even the most problematic DNA.
The revolutionary GC-RICH Solution, a PCR additive that reduces template secondary structure, is also supplied with this product.
•Conveniently use robotic pipetting systems.
PCR setup can be performed at room temperature due to the enzyme´s initial inactivity.
•Make PCR setup easier.
Manual hot start, wax barriers, beads, or the need to set up PCRs on ice can be eliminated when using this hot start enzyme.
•Prevent carryover contamination.
This enzyme allows the incorporation of modified nucleotides and dUTP. In combination with Uracil-DNA Glycosylase, it safeguards PCR from cross-contamination.
•Ensure optimal performance by using PCR-Grade Nucleotides.
Our high-purity nucleotides improve experimental consistency and sensitivity.
Product Description
The enzyme dNTPack comprises FastStart Taq DNA Polymerase and PCR-Grade Nucleotides in a ready-to-use solution. FastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified recombinant Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute heat activation step at 95°C. An optimized PCR buffer system and a GC-RICH Solution to enable the enzyme to handle a wide range of templates are also supplied.
Volume activity: 5 U/µl |