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FastStart High Fidelity PCR System
Blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein for high fidelity hot start PCR of DNA fragments up to 5 kb.
Contents
1.FastStart High Fidelity Enzyme Blend, (5 U/µl) in storage buffer
2.FastStart High Fidelity Reaction Buffer, 10x conc. with 18 mM MgCl2
3.FastStart High Fidelity Reaction Buffer, 10x conc. without MgCl2
4.MgCl2 Stock Solution, 25 mM
5.DMSO
Quality
Each lot of FastStart High Fidelity PCR System is function-tested in PCR. Quality control is performed routinely, using human genomic DNA and primers specific for a 1.8-kb fragment of the EPO gene and primers specific for a 4.8-kb fragment of the tPA gene.
Application
The FastStart High Fidelity PCR System – a new enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb.
Hot Start PCR
•Hot Start PCR
•Multiplex PCR
•RT-PCR
•GC-rich template amplification
•Difficult, challenging PCRs
Note: The FastStart High Fidelity PCR System is the product of choice for multiplex PCR. For more information, see the application note “Step-by-Step Protocol for Multiplex PCR with the FastStart High Fidelity PCR System and the PCR Optimization Kit ”. As this note demonstrates, the system amplifies up to 18 PCR targets simultaneously after the reaction is optimized with the PCR Optimization Kit.
Benefits
•Amplify longer templates.
The reagent enables the amplification of a variety of DNA and cDNA fragments up to 5 kb.
•Increase fidelity.
The enzyme blend shows a 4-fold higher fidelity compared to Taq DNA Polymerase and FastStart Taq DNA Polymerase.
•Achieve excellent performance in multiplex PCR.
The system amplifies multiple PCR fragments simultaneously. For difficult multiplex reactions, optimal performance is obtained in combination with our PCR Optimization Kit.
•Challenge even the most problematic DNA.
DMSO, a PCR additive that facilitates working with difficult templates, is conveniently supplied with this product.
Product Description
The FastStart High Fidelity PCR System is a unique blend of FastStart Taq DNA Polymerase and a novel thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below 75°C and are activated by heating to 95°C for 2 minutes.
Volume activity: 5 U/μl
For a standard 50 μl PCR, we recommend using 2.5 U of the enzyme blend. |