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Expand Long Template PCR System
For long and accurate amplifications from complex DNA templates from 5-20 kb by polymerase chain reaction (PCR).
Contents
1.Expand Long Template Enzyme Mix
2.Expand Long Template Buffer 1, 10x conc. with 17.5 mM MgCl2
3.Expand Long Template Buffer 2, 10x conc. with 27.5 mM MgCl2
4.Expand Long Template Buffer 3, 10x conc. with 27.5 mM MgCl2 and detergents
Application
Choose Expand Long Template PCR System for long and accurate PCR reactions. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20 kb in length. Even if Taq DNA Polymerase were able to amplify fragments of comparable lengths, Expand Long Template PCR System would deliver twice the fidelity and much higher yields.
•PCR
•RT-PCR
•Large-fragment amplification
Benefits
•Amplify longer templates than ever before.
This optimized enzyme blend and specially developed three-buffer set generate PCR products from 5 to 20 kb in length from complex genomic DNA.
•Achieve higher yields and fidelity.
The Expand Long Template PCR System delivers three times the fidelity and much higher yields compared to Taq DNA Polymerase.
•Improve PCR efficiency.
More full-length product is obtained when characterizing human gene loci, fingerprinting DNA, or isolating entire genes from cDNA or entire viral genomes.
Quality
Each lot of the Expand Long Template PCR System is function-tested in PCR using human genomic DNA and specific primers for a 9 kb, 12 kb, and 15 kb fragment. The enzyme mix is tested for the absence of any contaminating activities, including endo- or exonucleases and nicking activity. |