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Expand Long Range dNTPack
Next-generation kit for the consistent amplification of extra-long templates up to 25 kb from genomic DNA (or up to 40 kb from λDNA templates) by the polymerase chain reaction (PCR), with ready-to-use PCR Nucleotide Mix.
Contents
1.Expand Long Range Enzyme Mix
2.Expand Long Range Buffer with 12.5 mM MgCl2, 5× conc.
3.Expand Long Range Buffer without MgCl2, 5× conc.
4.MgCl2 Stock Solution, 25 mM
5.DMSO, 100%
6.PCR-Grade Nucleotide Mix (contains dATP, dCTP, dGTP, and dTTP, each at a 10 mM concentration).
Application
Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of thermostable DNA polymerases for use in diverse applications, including •Genome mapping and sequencing
•Contig construction
•Characterization of cloned sequences in lambda phages or cosmids
•Cloning and analysis of eukaryotic genes
•Rapid identification and cloning of complete genes from genomic DNA using cDNA sequence as starting material
•Two-step RT-PCR with templates (up to 12 kb) reverse transcribed with our Transcriptor First Strand cDNA Synthesis Kit
Benefits
•Consistently amplify long templates that other long-fragment systems can´t amplify.
•Amplify fragments up to 25 kb.
•Obtain high yields of your desired fragment - not nonspecific products.
•Perform a wide variety of applications by using an enzyme blend that has three times greater fidelity than Taq DNA Polymerase.
•Maximize performance, economy, and ease of ordering by obtaining high-quality enzymes, buffers, and nucleotide mix simultaneously in one cost-effective dNTPack.
•Save time and resources with a convenient and flexible product.
•Use one buffer to amplify all fragment sizes.
•Fine-tune your reaction using the supplied DMSO and MgCl2 solution, if needed.
Product Description
The Expand Long Range dNTPack is the second-generation version of the Expand Long Template PCR System, optimized and designed to consistently amplify PCR products up to 25 kb from genomic DNA (or up to 40 kb from λDNA templates). The supplied buffer can be used for all PCR product sizes. In addition, a buffer without MgCl2, and separate vials of MgCl2 and DMSO are included if optimization is needed. Expand Long Range dNTPack contains a unique enzyme mix that consists of thermostable Taq DNA polymerase and a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of PCR product. Due to the inherent 3´- 5´ exonuclease activity of the proofreading polymerase, Expand Long Range dNTPack copies DNA three-times more accurately than Taq DNA polymerase.
For a standard 50 µl PCR, we recommend using 3.5 U of the enzyme blend |