原装进口|Expand HiFi PCR Sys., 100 u

Expand High Fidelity PCR System  
Expand High Fidelity enzyme blend for extremly accurate amplification of genomic DNA targets up to 5 kb by polymerase chain reaction (PCR).
Contents
1.Expand High Fidelity Enzyme Mix
2.Expand High Fidelity Buffer, 10x conc. with MgCl2
3.Expand High Fidelity Buffer, 10x conc. without MgCl2
4.MgCl2 Stock Solution, 25 mM
Quality
Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1 kb amplified product) and the tPA gene (4.8 kb amplified product).
Application
The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length.
•PCR
•RT-PCR
Benefits
The Expand High Fidelity PCR System ensures improved results over Taq Polymerase when comparing yield, fidelity, and flexibility.
•Detect amplification products
that were previously undetectable and avoid false negatives.
•Achieve successful PCR results
from small quantities of template DNA.
•Achieve higher sensitivity
under a wider variety of conditions compared to Taq DNA Polymerase.
Product Description
For a standard 50 µl PCR, we recommend using 2.6 U of the enzyme blend.