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DNase I recombinant, RNase-free from bovine pancreas, expressed in Pichia pastoris
Application
Use this recombinant preparation of DNase I for:
•Producing DNA-free preparations of protein and RNA, e.g. to:
•Ensure RT-PCR templates are free of genomic DNA
•Remove DNA templates after in vitro transcription of RNA
•Nick-translation labeling of DNA (with added DNA polymerase I)
•Determining the "footprint" of a DNA-binding protein
•Microarray analysis
Benefits
•Eliminates DNA contamination from any RNA sample
•Contains no detectable RNase or protease activity
•Can be heat inactivated, thereby eliminating the need for organic extraction
•Shipped with an optimized incubation buffer, which supports maximum DNase activity
•Produced via an entirely animal-free process, to eliminate any risks associated with animal-derived material
Product Description
Brief description: Recombinant DNase I is a DNA-specific endonuclease. It randomly degrades both double- and single-stranded DNA to a mixture of oligo- and mononucleotides.
Source: bovine pancreas, expressed in Pichia pastoris
Molecular weight: Mr = approx. 39 kDa.
pH optimum: between pH 7.0 and 8.0
Heat inactivation: One unit of enzyme can be heat-inactivated by a 10 min incubation at 75°C.
Volume activity: 10 x 103 U/ml
Note: To determine volume activity, 100 µg calf thymus DNA is incubated in 2.5 ml incubation buffer with 20 to 50 units enzyme at 25°C. The absorbance increase is measured at 260 nm.
Unit definition: One unit of enzyme causes an absorbance increase of 0.001/min under assay conditions.
Incubation buffer (10x): 400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2; pH 7.9
Storage buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM MgCl2, 2mM CaCl2, 1 mM DTE, 0.1 mg/ml Pefabloc SC, 50% glycerol (v/v); pH 7.6 (at 4°C)
Dilution buffer: 25 mM Tris-HCl, 50% glycerol (v/v); pH 7.6 (at 4°C)
Contents
1.Recombinant DNase I, RNase-free, 10 U/μl (10 x 103 units)
2.Incubation Buffer, 10x conc. (5 x 1 ml)
Quality
Absence of contaminants: Each lot is tested to ensure the absence of RNases and proteases. |