T4 DNA Ligase recombinant form of the enzyme from T4 phageApplicationUse T4 DNA ligase to join sticky- or blunt-ended DNA fragments. Product DescriptionBrief description: T4 DNA ligase catalyzes the formation of phosphodiester bonds between neighboring 3´-hydroxyl and 5´-phosphate ends of double-stranded DNA. It will also close single-stranded nicks in double-stranded DNA. Source: E. coli NM989, acc. to Murray