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Dig nucleic acid detection kit
Principle
DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.
Application
The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic-acid hybrids.
Contents
DIG-labeled Control DNA, 50 µl, [5 µg/ml]
DNA Dilution Buffer, 1 ml, [50 µg/ml] fish sperm DNA
Anti-Digoxigenin-AP Conjugate, 200 µl, [750 U/ml]
NBT/BCIP, 8 x 1 ml, 50x stock solution
Blocking Reagent, 2 x 50 g |