在线咨询:点击这里给我发消息   点击这里给我发消息
旺 旺:欢迎随时给我旺旺留言
产品检索
品名:
货号:
品牌:
 
产品分类
  您现在的位置:产品中心 >> 分子生化试剂 >> 核酸标记和杂交

正品|DIG-High Prime DNA Labeling and Detection Starter Kit I(DIG High Prime Lab/Detection K)

产品编号: 11745832910     查看说明书
产品名称: 正品|DIG-High Prime DNA Labeling and Detection Starter Kit I(DIG High Prime Lab/Detection K)  .0   订购此产品 
供应商: Roche
规格: 1 kit (12 labeling reactions and 24 detection reac
目录价: 6135
库存状态: 大量现货
CAS编号:
应用范围: 高效DNA地高辛标记和检测试剂盒I
种属来源: Roche
相关信息:

Catalog Number: 11745832910
Pack size :1 kit for 12 labeling reactions and detection of 24 blots, 10 ng to 3 �g DNA per assay, blots of 100 cm2
品名:DIG-High Prime DNA Labeling and Detection Starter Kit I (高效DNA地高辛标记和检测试剂盒I )
检测方法:Kit for color detection with NBT/BCIP.

Contents
1.DIG-High Prime, 5x conc., 50 µl
2.DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)
3.DNA Dilution Buffer, 3 x 1 ml
4.Anti-digoxigenin-AP Conjugate, 100 µl
5.NBT/BCIP Stock Solution, conc., 6 x 1 ml
6.Blocking Solution, 10x conc., 4 x 100 ml
7.DIG Easy Hyb Granules, 4 x 100 ml
Application
DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.
Product Description
Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

 量大有特价!

 

保存条件: -25至-15℃
说明书地址: 点击查看详细
打印此页      关闭此页

上一个:现货|DIG-High Prime DNA Labeling and Detection Starter Kit II/DIG High Prime Lab./Det.Kit II[11585614910/1 kit (12 labeling reactions and 24 detection reac]
下一个:DIG Labeled Control DNA 50ul[11585738910/50 μl ]
电话:15821622703 15121098156 座机:021-54046790邮箱:sales@haoranbio.com info@haoranbio.com 地址:上海浩然生物(上海浩洋生物科技有限公司) QQ:470003480
 Copyright © 2009上海浩然生物(上海浩洋生物科技有限公司) All Rights Reserved. 网站备案:沪ICP备11023319号-1