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cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack
Protease Inhibitor Cocktail Tablets supplied in foil blister packs
Application
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows high inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore, metalloproteases are not inhibited.
For the purification of histidine-tagged proteins using Immobilized Metal Ion Affinity Chromatography (IMAC), this EDTA-free tablet is recommended for applications using non-EDTA compatible IMAC resins. Alternatively, an EDTA-containing cOmplete ULTRA, Mini tablet may be used in combination with Roche’s new EDTA-compatible chromatography material, cOmplete His-Tag Purification Resin.
Benefits
•Complete protection
The extended inhibitor cocktail composition makes this tablet an ideal formulation for your most difficult, protease-sensitive targets.
•Convenient tablet format
Eliminate weighing small amounts, and instantly protect your proteins against a now even wider range of proteases by simply pushing a tablet through the EASYpack foil packaging directly into your buffers.
•Suitable for IMAC purification
Purify poly-histidine tagged proteins using resins that are not compatible with EDTA. With Roche’s new EDTA-compatible cOmplete His-Tag Purification Resin, a tablet with EDTA may be used in IMAC, offering significant better protection of your target protein even against metalloproteases.
Product Description
cOmplete ULTRA Tablets, Mini, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA Tablet, Mini, EDTA-free is sufficient for a volume of 10 ml extraction solution.
Background Information
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. However, metalloproteases are prevalent in, for example, bacteria, and some techniques require that EDTA be omitted.
Obtain convenient, reliable protection of your proteins against dephosphorylation using the new PhosSTOP Phosphatase Inhibitor Cocktail Tablets. You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.
Contents
30 tablets individually packed in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution. |