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Collagenase D
Application
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures.
Collagenase D is recommended when functionality and integrity of cell-surface proteins are important.
Product Description
EC 3.4.24.3
Enzyme activity:
Collagenase activity: >0.15 U/mg (according to Wünsch) (25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase D has a normal to high collagenase activity and very low tryptic activity (usually <0.2 units/mg, BAEE as substrate).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.
Working concentration: 0.5 - 2.5 mg/ml |