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Cell Proliferation Reagent WST-1
Application
Use Cell Proliferation Reagent WST-1 to assay cell proliferation and viability of cell populations. Possible applications include:
•Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
•Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds.
•Assessment of antibodies and physiological mediators that inhibit cell growth.
Note: The WST-1 assay is nonradioactive, spectrophotometric, and performed entirely in 96-well plates.
Benefits
•Safe, since the assay does not use radioactive isotopes.
•Accurate, since assay absorbance strongly correlates to the cell number.
•Sensitive, since the assay can detect even low cell numbers.
•Fast, since the assay can be read in a multi-well ELISA reader, which allows simultaneous processing of many samples.
•Easy, since the assay does not require any washing steps or additional reagents.
•Convenient, since the assay reagent is ready to use.
Product Description
Sample material: Adherent or suspension cells cultured in 96-well microplates.
Background Information
There are several colorimetric assays that can analyze the number of viable cells; each is based on the cleavage of tetrazolium salts that are added to the culture medium. These assays do not require either washing or harvesting of cells. The complete assay, from microculture to data analysis, can be performed in the same microplate.
Contents
Ready-to-use solution, containing WST-1 and an electron coupling reagent |