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Biotin RNA Labeling Mix
Application
This labeling mix can be used (with SP6, T3, or T7 RNA polymerase) to add a nonradioactive label to RNA during in vitro transcription. The labeled RNA can easily and safely be detected (e.g ., with a fluorescein-conjugated or enzyme-conjugated streptavidin).
Product Description
Brief description
The ready-to-use, 10x conc. mix contains:
•ATP, CTP, and GTP, each at a concentration of 10 mM •UTP, 6.5 mM •biotin-16-UTP, 3.5 mMLabeling Efficiency
A standard labeling reaction with 1 µg linear template DNA and an RNA polymerase produces approx. 10 µg of full-length, biotin-labeled RNA. In a spot test, a combination of anti-biotin-AP and the chemiluminescence substrate CSPD can detect as little as 0.3 pg of the biotin-labeled RNA.
Background Information
The biotin-labeled RNA can be used as a hybridization probe for:
•Northern blots •Southern blots •Plaque or colony lifts •RNase protection experiments •In situ hybridization •Microarray hybridization
Contents
Solution, 10x conc., pH 7.5 |