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Application
Protection of proteins and enzymes during isolation/purification.
Purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin.
Quantification of kallikrein activity in mixtures of esterases and proteases.
Inhibition of protease activities that can shorten the lifetime of cells in cell and tissue culture.
May be used as a model protein in protein-folding studies.
Molecular weight marker in SDS-polyacrylamide gel electrophoresis.
Product Description
Other name: Pancreatic trypsin inhibitor (BPTI)
Activity: approx. 630 U/mg (25°C, Chromozym TRY as the substrate) = approx. 200 U/mg (trypsin inhibitor units, 25°C, BAEE as the substrate)
Specificity: Highly specific inhibition of serine proteases such as plasmin, kallikrein, trypsin, and chymotrypsin. Does not act on thrombin or factor X.
Working concentration: 0.06 to 2 µg/ml (0.01 - 0.3 µM)
Solubility/Stability: Freely soluble in water (10 mg/ml) or aqueous buffer solution (e.g., Tris, 0.1 M, pH 8.0). A solution adjusted to pH 7–8 is stable for approximately 1 week at +2 to +8°C. Aliquots stored at -15 to -25°C are stable for approximately 6 months.
Note: Avoid repeated freezing and thawing and exposure to strongly alkaline solutions (inactive at pH >12.8).
Contents
Lyophilizate
Quality
Purity: Electrophoretically homogeneous
Aprotinin from bovine lung
10981532001 50 mg | 名称 | 产地 | 货号 | 规格 | 价格 | | Aprotinin from bovine lung | ROCHE | 10981532001 | 50MG | 2698 | |