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Application
The Apoptotic DNA-Ladder Kit provides rapid isolation of DNA, which can be analyzed and characterized by gel electrophoresis for the determination of apoptotic cell death.
Background Information
The study of cell death involves characterizing mortality as apoptotic or necrotic. Apoptosis can be characterized by:
Prelytic, non-random fragmentation of DNA (“ladder” pattern after agarose-gel electrophoresis)1
Formation of membrane-bound vesicles (or “apoptotic bodies”)
Cell shrinkage due to a concentration of cytoplasm
Similarly, necrosis (or physiological cell death) is characterized by
Random digestion of DNA (DNA smear after agarose-gel electrophoresis)
Swollen organelles and cells, resulting from loss of membrane integrity and cell lysis
Postlytic DNA fragmentation
As these differences indicate, the classification of cell death can be accomplished through observing cell morphology, or less subjectively, by analyzing genomic DNA. Resolving the DNA on a gel provides a quick and documentable form of data to differentiate between apoptosis and necrosis.
1. Wyllie, A. H. (1980) Nature 284, 555.
Contents
Binding/Lysis Buffer, 20 ml
Washing Buffer, 20 ml
Elution Buffer, 40 ml
Polypropylene Tubes that contain two layers of glass fiber fleece, and can hold up to 700 µl sample volume; 2 bags with 10 Filter Tubes
Polypropylene Collection Tubes (2 ml); 2 bags with 10 Collection Tubes
Control Apoptotic U937 Cells, lyophilized
Principle
Blood or cell lysis is accomplished by incubating the sample with the special Binding/Lysis Buffer. The sample is centrifuged through a column that contains glass fiber fleece. In the Binding/Lysis Buffer, nucleic acids quickly and preferentially bind to the surface of the glass fibers. The Washing Buffer rinses away salts, proteins, and other cellular debris. DNA is subsequently collected via a centrifugation step with elution buffer.
Apoptotic DNA-Ladder Kit
11835246001 1 kit for up to 20 tests | 名称 | 产地 | 货号 | 规格 | 价格 | | Apoptotic DNA-Ladder Kit | ROCHE | 11835246001 | 20T | 4091 | |