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Anti-GFP from mouse IgG1κ (clones 7.1 and 13.1)
For the detection of GFP (green fluorescent protein) -fusion proteins.
Application
Anti-GFP can be used to verify the expression of Green Fluorescent Protein (GFP) and GFP fusion proteins by western blot analysis. It also works well for the immunoprecipitation of GFP and GFP fusion proteins. Anti-GFP recognizes both wild-type and mutant forms of GFP.
For life science research only. Not for use in diagnostic or therapeutic procedures.
Product Description
Subtype: Both clones are Mouse IgG1κ
Background Information
Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27- kDa protein originally isolated from the jellyfish Aequorea victoria (1). The molecular cloning of the GFP gene (2) and its subsequent expression in heterologous systems (3) has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since GFP requires no additional substrates or cofactors, GFP’s green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported (4,5).
References
1.Ward, W.W., Cody, C.W., Hart, R.C. and Cormier, M.J. (1980) Photochem. Photobiol. 31, 611–615.
2.Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G. and Cormier, M.J. (1992) Gene 111, 229–233.
3.Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C. (1994) Science 263, 802–805.
4.Cormack, B.P., Valdivia, R., and Falkow, S. (1996) Gene 173, 33-38.
5.Crameri, A., Whitehorn, E.A., Tate, E. and Stemmer, W.P.C. (1996) Nature Biotechnology 14, 315–319.
Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200 μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Quality
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity
Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
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