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Application
For the detection of fluorescein-labeled compounds.
ELISA
Immunohisto/cytochemistry
In situ hybridization
Western blot
Product Description
Clone: B13-DE1
IgG class: mouse IgG1
Specificity: The monoclonal antibody (clone B13-DE1) reacts with free and bound fluorescein.
Working concentration:
ELISA: 2 - 4 µg/ml
Immunohisto/cytochemistry: 0.5 - 2 µg/ml
In situ hybridization: 0.2 - 0.4 µg/ml
Western blot: 0.5 - 2 µg/ml
Background Information
To obtain monoclonal antibodies, Balb/c mice were immunized with KLH-bound fluorescein and the spleen cells were subsequently fused with myeloma cells of the cell line NS-1. The antibodies were purified by gel filtration, diluted in 10 M potassium phosphate buffer, 75 mM NaCl, 2% raffinose (w/v), 0.01% 2-methylisothiazolone (MIT) (w/v), pH 7.4, and subsequently lyophilized.
Contents
Lyophilizate, stabilized
Anti-Fluorescein from mouse IgG1 (clone B13-DE1)
For the detection of fluorescein-labeled compounds.
11426320001 100 µg | 名称 | 产地 | 货号 | 规格 | 价格 | | Anti-Fluorescein from mouse IgG1 (clone B13-DE1) | ROCHE | 11426320001 | 100ug | 3199 | |