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Anti-Bromodeoxyuridine from mouse IgG1(clone: BMC9318)
For monitoring of cell proliferation.
11170376001 50 µg 500 µl
Application
Cryosections, Immunochemistry, Flow cytometry, Paraffin sections
Anti-Bromodeoxyuridine monoclonal antibody can be used for identifying proliferating cells in blood, tissues, and tumors, as well as for determining plasma cell labeling indices.
Product Description
Clone:
BMC9318
Ig class:
mouse IgG1
Preparation:
BALB/c mice were immunized with a bromodeoxyuridine-bovine serum albumin conjugate. Lymphocytes isolated from the spleen were fused with Ag8.653 myeloma cells to create the BMC 9318 clone. The antibody was produced in ascites in BALB/c mice and purified by ion-exchange chromatography.
No. of tests:
250 (Flow cytometry)
Specificity:
The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromo-deoxyuridine does not crossreact with fluorodeoxy-uridine, nor with any endogenous cellular components such as thymidine or uridine.
Working concentration
Use approximately 0.2 μg/100 μl/106 cells for flow cytometry and 6 μg/ml for immunohistochemistry. These are guidelines only. The optimal concentration should be determined by the investigator. Dilutions should be made in PBS (pH 7.4) containing 0.1% BSA to maintain stability of the antibody.
Background Information
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells undergoing replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, antibromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.
Contents
50 µg Anti-bromodeoxyuridine is supplied in 500 µl PBS (phosphate buffered saline), pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin for stability.
Quality
The antibody is ≥90% pure as determined by SDS-PAGE with Coomassie-blue staining, and by HPLC | 名称 | 产地 | 货号 | 规格 | 价格 | | Bromodeoxyuridine | ROCHE | 11170376001 | 50ug | 5200 | |