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Application
The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using Annexin-V-FLUOS. Additionally, labeling with propidium iodide (PI) or with a cell surface marker can be used for differentiation from necrotic cells.
Product Description
Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.
Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine.
Background Information
In the early stages of apoptosis, changes occur on the cell surface (e.g., translocation of phosphatidylserine [PS] from the interior side of the plasma membrane to the outer leaflet). This secretion of phosphatidylserine is the ideal basis for Annexin-V, which binds to PS with high affinity. Annexin-V is a Ca2+-dependent phospholipid-binding protein with a high affinity for PS. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain, which is used for dye-exclusion testing, allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.
Contents
500 µl, ready-to-use solution, for 250 tests
Annexin-V-FLUOS
11828681001 500 µl 250 tests | 名称 | 产地 | 货号 | 规格 | 价格 | | Annexin-V-FLUOS | ROCHE | 11828681001 | 250t | 5868 | |