|
Agarose Gel DNA Extraction Kit
For the elution of DNA fragments from agarose gels
11696505001 1 kit (for max. 100 reactions)
Notes
By combining agarose gel electophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 - 50 μl.
Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. For example, recovered DNA can be efficiently ligated into cloning vectors or labelled to high specific activity. Restriction digests proceed without inhibition.
Quality
DNA Molecular Weight Marker II is separated in a 0.8% agarose gel and the 546-, 2322-, and 6557-bp fragments are isolated with the kit components. The expected amount of DNA is recovered for each fragment. Depending on parameters such as fragment length, preparation, and purity of the applied DNA, up to 80% recovery is achieved. DNA fragments are digested with restriction enzymes. No inhibition of DNA digestion is observed
Application
The kit can efficiently isolate both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for:
Ligation and transformation
Enzymatic restriction
Labelling by random primed or nick translation methods
Sequencing
Benefits
Quick and simple; extraction procedure requires approx. 45 min time and only a few hands-on steps.
Works with different agaroses and buffer systems; low melting point agarose is not required.
Efficient; specific binding of DNA allows easy removal of impurities.
Isolates large DNA fragments without shearing; silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments (up to 100 kb).
Even isolates oligonucleotides (≥20 bp); narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matrix.
Free of fines and small particles; recovered product will not inhibit subsequent enzymatic reactions.
Good yield; see "Product Description" for typical recoveries.
Product Description
Sample size: 100 mg gel slice (for 1 standard reaction)
Size range of recovered DNA: 20 bp - 100 kbp
Typical recovery: 20 bp, 55%; 40 bp, 68%, 120 bp, 76%; 200 bp, 80%; 8 - 9 kb, 75%; larger than 10 kb, <60%
Contents
Silica Matrix, for 100 standard reactions
Solubilization Buffer, 60 ml
Nucleic Acid Binding Buffer, 100 ml
Wash Buffer, 20 ml | 名称 | 产地 | 货号 | 规格 | 价格 | | Agarose Gel DNA Extraction Kit | ROCHE | 11696505001 | 盒 | 1597 | |