|
Product Description
7-deaza-2'-deoxyguanosine-5'-triphosphate, Li+ salt
Formula: C11H17N4O13P3
Molecular weight: 506.2
Form: clear, colorless solution in water
Concentration: 10 mM
pH: 7
Stability: stable for 12 months after the production date when stored at -15 to -25°C
Contents
Lithium salt solution, 10 mM, pH 7
Quality
Typical analysis: Preparation contains at least 95% 7-deaza-dGTP.
Notes
When PCR products contain 7-deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-deaza-dGTP.
Application
The incorporation of 7-deaza-2'-deoxyguanosine-5'-triphosphate (7-deaza-dGTP) into DNA can eliminate spurious GC-hydrogen bonding and relax secondary structures, an effect that may be useful in all types of dideoxy sequencing and polymerization techniques. For example:
Sequencing: Use 7-deaza-dGTP) instead of dGTP in primer extension reactions (e.g., Sanger sequencing) to better resolve GC-rich regions on gels.
PCR: Partial substitution of 7-deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures) | 产品名称 | 产地 | 货号 | 规格 | 价格 | | 7-Deaza-2'-deoxy-guanosine-5'-triphosphate | ROCHE | 10988537001 | 200UL | 1845 | |