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7-Deaza-2'-deoxy-GTP, Di-Li

产品编号: 10988537001     查看说明书
产品名称: 7-Deaza-2'-deoxy-GTP, Di-Li  .0   订购此产品 
供应商: Roche
规格: 2 μmol (200 μl)
目录价: 1898
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Product Description
7-deaza-2'-deoxyguanosine-5'-triphosphate, Li+ salt
Formula: C11H17N4O13P3
Molecular weight: 506.2
Form: clear, colorless solution in water
Concentration: 10 mM
pH: 7
Stability: stable for 12 months after the production date when stored at -15 to -25°C
Contents
Lithium salt solution, 10 mM, pH 7
Quality
Typical analysis: Preparation contains at least 95% 7-deaza-dGTP.
Notes
When PCR products contain 7-deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished. 
PCR amplification efficiency is generally not affected by 7-deaza-dGTP.
Application
The incorporation of 7-deaza-2'-deoxyguanosine-5'-triphosphate (7-deaza-dGTP) into DNA can eliminate spurious GC-hydrogen bonding and relax secondary structures, an effect that may be useful in all types of dideoxy sequencing and polymerization techniques. For example:
Sequencing: Use 7-deaza-dGTP) instead of dGTP in primer extension reactions (e.g., Sanger sequencing) to better resolve GC-rich regions on gels.
PCR: Partial substitution of 7-deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures)
产品名称 产地 货号 规格 价格
7-Deaza-2'-deoxy-guanosine-5'-triphosphate ROCHE 10988537001 200UL 1845

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