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产品名称：TOPO® TA Cloning® Kit for Subcloning without Competent Cells
产品货号：450641
产品规格：20个反应
产品价格：5790
Contents and Storage
This kit contains linearized and topoisomerase I-activated pCR®2.1 TOPO® Vector, Salt Solution,
dNTPs, Control Template and Primers, and M13 Forward and Reverse Primers.Store all components at -20 deg;C. All reagents are guaranteed stable for 6 months when properly stored
Specifications
General Specifications
PCR Enzyme: Taq Polymerase
Vector Type: pCR,
TOPO-TA Cloning Vectors
Product Size: 20 reactions
Cloning Method: TOPO®-TA
Directionality: No
Replication Origin: f1 Origin,
pUC Origin
Bacterial or Yeast Strain: Not Included
Antibiotic Resistance (Bacterial): Kanamycin (KanR),
Ampicillin (AmpR)
The TOPO® TA Cloning® Kits for Subcloning provide a highly efficient,
5 minute,
one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for
subcloning. Each kit uses the pCR™2.1-TOPO® TA vector with convenient restriction sites for subcloning and
are available with a variety of competent cells, or no competent cells, depending on your needs and budget.
Fast and Easy—Go from PCR-to-clones in just 3 steps and in as little as 5 minutes hands-on time
Efficient—Achieve up to 95% clones with correct insert
Proven—Reliable performance for over a decade with over 4000 citations
Simple —No ligase, post-PCR procedures, or PCR primers containing specific sequences are required.
TOPO® TA Cloning® Kits for Subcloning Kit Overview
VECTOR: pCR™2.1-TOPO® TA Vector
— Subcloning vector with 15 convenient and validated restriction sites flanking your insert for easy, directional sub-cloning
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