5' RACE System for Rapid Amplification of cDNA Ends, Version 2.0 2.0版5´ RACE系统适用于cDNA末端的快速扩增 (RACE) (1) 及mRNA中确定点和5´ 末端之间的锚式PCR (2)。该系统可用于扩增序列信息较少的罕见mRNA,且可用于捕获mRNA的5´ 末端信息 (3,4)。5´ RACE系统: ■利用SuperScript® II RNase H- RT的先进性能,提高第一链cDNA产量,增加cDNA长度 ■在一个反应中,利用至多1 µg的总RNA或poly(A)+ RNA制备特定的cDNA ■提供更完善的第一链cDNA纯化步骤,获得适合加尾的cDNA ■在与PCR相容的缓冲液中,以优化的反应条件使用重组TdT,为PCR提供高效的引物退火位点 (图1) Product Size: 10 reactions Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. The 5´ RACE System is supplied with the components listed in Table 1. Store the reagents at -20°C and the DNA purification components at +4°C. All reagents guaranteed stable for 6 months when properly stored.