ElectroMAX™ DH12S™ Cells ElectroMAX™ DH12S™细胞为DH10B™的衍生物，适于电转化应用 (1,2)。可用于生成单链DNA，或用于制备cDNA、基因组及消减cDNA文库等要求高转化效率的操作中。DH12S™细胞株带有endA+基因，因而可以生成较少双链DNA污染的ssDNA。标记F´上lacIq基因使得lac启动子的可调控基因表达。与基因组DNA克隆中使用的多数大肠杆菌宿主不同 (3)，限制DNA中胞嘧啶和腺嘌呤甲基化的基因系统（mcrA、mcrB、mcrC和mrr）已经从DH12S™细胞株中消除，因此能够构建更具代表性的基因组文库或者更有效的质粒拯救操作 (4,5)。还提供了M13KO7辅助噬菌体以便制备ssDNA。ElectroMAX™ DH12S™细胞能提供： ■电转化效率>1 x 1010转化子/µg，带来无与伦比的克隆结果 ■独特的基因型，支持生产特别纯的单链DNA ■稳定的F´附加体，无需在基本培养基上生长 ■消除了mcrA，mcrBC，mrr，hsdRMS等限制系统，能获得更好的序列代表性 ■带有lacZΔM15，重组克隆中可以进行蓝白斑筛选 ■即用型M13KO7辅助噬菌体可用来生成单链DNA Competent Cell Type: Electrocompetent Species: E. coli Format: Tube(s) Transformation Efficiency: > 1x10^10 Bacterial or Yeast Strain: DH12S™ Plasmid Type: May be used for plasmids > 20 kb Product Size: 0.5 mL Blue-White Screening: Yes Cloning Unstable DNA: Not Suitable for cloning unstable DNA Reduces Recombination: Yes Cloning Methylated DNA: Yes Improves Plasmid Quality: No Propagating ccdB Vectors: Not for ccdB vector propagation F' Episome (to make ssDNA): Contains F' Episome Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA T1 Phage - Resistant (tonA): No High Throughput Compatibility: Not High Throughput-Compatible Shipping Condition: Dry Ice Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. Store in ultra-cold freezer (-68 to -85°C).