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MAX Efficiency® DH5α™ Competent Cells
DH5α™ 是有名的多用途菌株，可以在很多日常克隆应用中使用。 除了支持蓝/白筛选外，DH5α™ 中的 recA1 和 endA1 突变能增加插入片段的稳定性，提高通过小量提取制备的质粒 DNA 的质量。 DH5α™ 细胞有以下优点：
■提供各种范围的效率，从 >1 x 106 到 >1 x 109 个转化体/µg
■通过 endA1 突变显著增加质粒的产量和质量
■利用 lacZΔM15 进行重组克隆蓝/白筛选
■通过 recA1 突变保证了插入片段的稳定性
Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: DH5α™
Product Size: 10 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Contains:
• Max Efficiency® DH5α™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |