DNA Polymerase I/DNase I DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA. Application: Labeling DNA with either radiolabeled or biotinylated nucleotides. Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964. Performance and Quality Testing: Performance tested in nick translation reaction. Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer. Hot Start: No Polymerase: DNA Polymerase I Product Size: 250 units Exonuclease Activity: 3' - 5', 5' - 3' Shipping Condition: Wet Ice Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.