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原装现货|SUPERSCRIPT III REV TRANSCRIPT
描述:
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.
Thermostability – Half life of 220 minutes at 50°C
Yield – Reduced RNase H activity for more full-length cDNA
Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
应用:
Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE
详细说明:
Optimal Reaction Temperature: 50° C
Final Product Size(s): 12.3 kb or less
Ribonuclease H Activity: Reduced
Concentration: 200 units/µl
Form: Frozen
Format: Tube(s)
Quantity: 2000 units
Template: ssRNA
Sensitivity: Medium
Product Size: 2,000 units
Enzyme Function: RNA dependent DNA Polymerase
Number of Reactions: 10 Reactions
内容和储存:
SuperScript® III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored. |