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Pfx50™ DNA Polymerase
Pfx50™ DNA Polymerase is a designer enzyme derived from the archaean Thermococcus zilligii. In addition to 3´ to 5´ proofreading ability, Pfx50™ polymerase is fused to an accessory protein for enhanced fidelity and processivity. This unique combination makes the Pfx50™ enzyme ideal for applications that require the highest fidelity and PCR success the first time. With Pfx50™ DNA Polymerase, you will:
• Achieve ultra low error rates—50 times better than Taq, without compromising PCR robustness
• Save time and money—highest fidelity means youll sequence fewer clones to obtain error-free constructs
• Produce results quickly and easily—robust performance with extremely short extension holds
Downstream compatibility with popular cloning techniques
Pfx50™ DNA Polymerase produces blunt-ended PCR products for compatibility with Directional TOPO® Expression Kits and Zero Blunt® TOPO® PCR Cloning Kits.
Polymerase: Pfx50™ DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 50 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 4 kb or less
Product Size: 500 reactions
Exonuclease Activity: 3' - 5'
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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