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AccuPrime™ Taq DNA Polymerase, high fidelity
AccuPrime™ Taq DNA Polymerase, High Fidelity, amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase, High Fidelity, you'll get:
• The highest specificity and yield for the most robust PCR amplification
• 9-fold higher fidelity than Taq DNA polymerase alone
• Mimimal optimization steps, even with non-optimized primer sets
• Efficient amplification of targets over a broad size range up to 20 kb
How it works
High fidelity is achieved by a combination of Platinum® anti-Taq DNA polymerase antibodies that inhibit polymerase activity, providing an automatic "hot-start", and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D. The thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.
Polymerase: AccuPrime™ Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 9 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A,
Mixed
Reaction Speed: Standard
Final Product Size(s): 20 kb or less
Product Size: 1,000 reactions
Exonuclease Activity: 3' - 5',
5' - 3'
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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