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AccuPrime™ GC-Rich DNA Polymerase
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B). Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Polymerase: AccuPrime™ GC-Rich DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 2 X
GC-Rich PCR Performance: High
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 1,000 reactions
Exonuclease Activity: 3' - 5'
Shipping Condition: Approved for shipment on Wet or Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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