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Cloned AMV First-Strand cDNA Synthesis Kit
The Cloned AMV First-Strand Synthesis Kit combines the high performance of Invitrogen™ Cloned AMV RT with the convenience of optimized, pre-qualified reagents. The and ! subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen™ Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum® Taq DNA Polymerase for high-specificity PCR or with Platinum® Taq DNA Polymerase High Fidelity for subsequent PCR cloning.
The Cloned AMV First-Strand cDNA Synthesis Kit:
■Uses high-purity Cloned AMV for higher yields
■Includes 5X first-strand buffer with proprietary agents for more robust performance
■Replaces the cDNA Cycle® Kit and AMV Reverse
Transcriptase System
■Detects transcripts in 1.0 pg of RNA (Figure 1)
■Detects various mRNA targets ranging in size from 1.6 kb to 9.4 kb (Figure 2)
Reverse Transcriptase: AMV (Recombinant)
Downstream Application: RT-PCR (Endpoint)
Reaction Format: Separate Components
Form: Frozen
Format: Tube(s)
Quantity: 1500 units
Template: ssRNA
Sensitivity: Low
Product Size: 100 reactions
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s): 9.4 kb or less
Optimal Reaction Temperature: 45-55° C
The Cloned AMV First-Strand cDNA Synthesis Kit is supplied with the components listed in Table 1. Store at -70°C. Guaranteed stable for 6 months when properly stored. |