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AMV Reverse Transcriptase, cloned
AMV Reverse Transcriptase, Cloned, is used to synthesize first-strand cDNA for amplification by PCR or for cloning subsequent to conversion to double-stranded cDNA. Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV. The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb. AMV RT has endoribonuclease H activity.
Features of this enzyme:
• Flexibility—works with oligo(dT), random primers, or gene-specific primers
• Thermostability—AMV is active at temperatures up to 55°C
• Multiple applications—first-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA
Source
Purified from insect cells infected with recombinant baculovirus expressing AMV pol.
Optimal Reaction Temperature: Up to 55° C
Final Product Size(s): 12.3 kb or less
Reverse Transcriptase: AMV (Recombinant)
Ribonuclease H Activity: Yes
Format: Tube(s)
Quantity: 750 units
Template: ssRNA
Sensitivity: Low
Product Size: 750 units
Concentration: 15 U&⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice
Number of Reactions: 50 Reactions
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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