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Gateway® pMT-DEST48 Vector
The pMT-DEST48 destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent expression in Drosophila S2 cells. As part of the DES® Expression System, pMT-DEST48 uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT-DEST48 vector offers the following features:
■C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
■C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
Promoter: MT
Vector Type: pDEST,
pMT
Product Size: 6 µg
Cloning Method: Gateway®
Protein Tag or Fusion: His Tag (6x),
V5 Epitope Tag
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
The pMT-DEST48 Gateway® destination vector is supplied supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored. |