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CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Features of the CellsDirect™ One-Step qRT-PCR Kit include:
• Efficiency—Offers significant time and cost savings for increased throughput
• Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
• Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add
PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Fast,
Standard
Platform: 7300 System,
7900HT Fast System,
Eppendorf MasterCycler RealPlex,
7000 System,
7900HT System,
7700 System
Sample Type (General): Cells
Product Size: 100 reactions
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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